FastQCFastQC Report
Fri 20 Mar 2015
lane7_Undetermined_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane7_Undetermined_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6743403
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG102900.015.38754751
TTTGTGT115250.013.4803352
TTGTGTT132250.012.1383973
TAACTTT58700.011.1179481
TGTGTTT147050.010.8537674
GTGTTTG148950.010.6154355
AACTTTG61200.010.5833612
ACTTTGT85050.09.9651341
TGTTTGA169650.09.2551636
GTTTGAG88500.08.5962957
TTTGAGG39200.07.56005248
CACGGTA9352.0008883E-116.841507445
GTTTGAC63100.06.55402957
CTCACGG9856.311893E-106.199506843
TTGAGGG28950.06.11466039
TCACGGT10757.767085E-105.901661444
GTTTGAT68150.05.8736617
TTTGAGT46450.05.6658798
CCTCACG10755.353286E-95.66328742
CCCTCAC12602.401066E-105.536335541
Produced by FastQC (version 0.11.2)