FastQCFastQC Report
Fri 20 Mar 2015
lane6_Undetermined_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane6_Undetermined_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7744903
Sequences flagged as poor quality0
Sequence length51
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT72300.013.3786772
TAACTTT47900.012.2135511
CTTTGTG82100.011.8410311
TTGTGTT86650.011.5174223
AACTTTG53400.010.6647712
TGTGTTT93700.010.6454314
GTGTTTG96650.010.358185
TGTTTGA112050.08.6636666
ACTTTGT70400.08.4623133
CGGTGTG12250.07.81063817
GTGTACA14000.07.72063521
TGTGTAC14200.07.61136620
GCGGTGT12850.07.413522716
TCACGGT14100.07.244045744
GTTTGAC68750.06.97807557
CTCACGG15150.06.877154443
CACGGTA13800.06.7919545
GGTGTGT16450.06.40233418
GTTTGAG65950.06.07307247
TGACGGG19600.06.07121310
Produced by FastQC (version 0.11.2)