FastQCFastQC Report
Fri 20 Mar 2015
L2V18DR-BC9_AAGAGG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL2V18DR-BC9_AAGAGG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37265508
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG670600.014.1766271
TTTGTGT711800.013.2557242
TAACTTT349100.012.1814081
TTGTGTT849350.011.5813873
AACTTTG377600.011.4567532
GTGTTTG916450.010.73059755
TGTGTTT942100.010.5009284
ACTTTGT511400.010.4856761
TGTTTGA1044000.09.4049786
GTTTGAG553500.08.5713647
TTTGAGG253300.07.4098868
TCCGATC71200.07.04890877
GTTTGAC344450.06.82136547
CCGATCT75600.06.6384058
GTTTGAT399900.06.52835237
TTTGAGT232450.06.3511888
CACGGTA61750.05.68276445
CTTCCGA88000.05.62717775
CTCACGG63750.05.50476943
TTGAGTG151100.05.2718139