FastQCFastQC Report
Fri 20 Mar 2015
L2V18DR-BC8_GTCGTA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL2V18DR-BC8_GTCGTA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44898514
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG813650.014.7385251
TTTGTGT865050.013.81878952
TTGTGTT1045350.011.8816743
TAACTTT422450.011.5263061
GTGTTTG1146300.010.8369285
TGTGTTT1157650.010.836014
ACTTTGT620550.010.426031
AACTTTG477750.010.3270142
TGTTTGA1316050.09.3949526
GTTTGAG658700.08.8629257
GTTTGAC401500.06.95634277
TTTGAGG312700.06.9449958
TTTGAGT281250.06.31330738
GTTTGAT550100.06.26775267
TTTGAGC207600.05.4635598
TTGAGTG178700.05.2886349