FastQCFastQC Report
Fri 20 Mar 2015
L2V18DR-BC6_GTGCTT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL2V18DR-BC6_GTGCTT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34218896
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT252050.015.87974551
AACTTTG274200.014.5869932
TTTGTGT448600.014.3801272
CTTTGTG533800.012.2466291
TTGTGTT587900.011.4569173
TGTGTTT643400.010.5665114
GTGTTTG654900.010.4840365
ACTTTGT406700.09.8660133
TGTTTGA781800.08.7129286
GAGGCTA55250.07.89933414
AGGCTAC56950.07.701842315
CTACCCG60100.07.561115318
GCTACCC61400.07.327706317
CACGGTA78650.07.094434745
GTTTGAG418350.06.9390987
CTCACGG83650.06.913228543
GTTTGAC438600.06.86500077
TCACGGT87700.06.61888744
TGACGGG93200.06.494157310
CCTCACG91550.06.36516142
Produced by FastQC (version 0.11.2)