FastQCFastQC Report
Fri 20 Mar 2015
L2V18DR-BC5_ACCTCA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL2V18DR-BC5_ACCTCA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39473106
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT265800.013.6111161
TTTGTGT401750.013.3405452
AACTTTG282600.012.7921472
CTTTGTG518800.010.5231221
TTGTGTT536700.010.5081293
TGTGTTT575850.09.8130734
GTGTTTG578050.09.7601285
ACTTTGT415200.08.9542383
TGTTTGA672750.08.3459286
GAGGCTA60550.07.393457414
AGGCTAC64300.07.03145715
CTACCCG66800.07.004769318
GCTACCC70650.06.75038417
GTTTGAC441000.06.4093317
TGACGGG93150.06.40084210
GGTGCGC26050.06.1350862
TACTAAG79700.06.09866841
TTGACGG110700.06.057539
GTTTGAG368150.05.98438457
GACGGGC89900.05.955892611
Produced by FastQC (version 0.11.2)