FastQCFastQC Report
Fri 20 Mar 2015
L2V18DR-BC4_GCACTA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL2V18DR-BC4_GCACTA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39526979
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT420600.012.9669822
TAACTTT271200.012.9078791
AACTTTG302400.011.5000972
CTTTGTG528750.010.5119891
TTGTGTT548350.010.4367893
GTGTTTG593750.09.7486915
TGTGTTT593900.09.6666884
ACTTTGT431600.08.2386113
TGTTTGA702450.08.1855186
GAGGCTA62700.07.391066614
AGGCTAC63450.07.196563715
CTACCCG65950.07.09497818
GCTACCC68500.06.92936317
TGACGGG100950.06.864788510
GTTTGAC408000.06.7237837
TTGACGG117950.06.44840969
CGGTGTG88200.06.274338217
ACGGGCG90500.06.239347512
CTAAGCG75350.06.18154943
GACGGGC95750.06.17941711
Produced by FastQC (version 0.11.2)