FastQCFastQC Report
Fri 20 Mar 2015
L2V18DR-BC3_AGTGAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL2V18DR-BC3_AGTGAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40215941
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT278700.013.6509831
AACTTTG306150.012.4693762
TTTGTGT437300.012.3585012
CTTTGTG544300.010.2199461
TTGTGTT574650.09.88462453
GTGTTTG615000.09.4190265
TGTGTTT614550.09.3453844
ACTTTGT447450.08.6961043
TGTTTGA727150.07.9475816
GAGGCTA59300.07.435558314
AGGCTAC62500.07.05389315
CTACCCG66200.06.830320418
TGACGGG95450.06.48234510
GCTACCC67550.06.427378717
GTTTGAC430050.06.07546337
GTTTGAG394950.06.00001577
TTGACGG111700.05.98329269
TACTAAG73350.05.98228541
TGGGCCC39850.05.92808245
CTAAGCG73000.05.856658543
Produced by FastQC (version 0.11.2)