FastQCFastQC Report
Fri 20 Mar 2015
L2V18DR-BC2_TGGTGA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL2V18DR-BC2_TGGTGA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43975164
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT315800.014.0077571
TTTGTGT529550.013.7765772
AACTTTG332850.013.4983652
CTTTGTG636350.011.6355261
TTGTGTT698300.010.9993283
TGTGTTT749450.010.3057234
GTGTTTG768800.010.063895
ACTTTGT495500.09.1608153
TGTTTGA907100.08.5541736
GAGGCTA80000.07.64898114
TGACGGG118050.07.357235410
CTACCCG84900.07.31333118
AGGCTAC80750.07.270509715
GCTACCC88350.07.15505717
GTTTGAC524200.06.8046557
GACGGGC108100.06.63901311
TTGACGG139800.06.58354579
ACGGGCG105050.06.424700312
GTTTGAG471250.06.41355047
CGGTGTG107650.06.3738117
Produced by FastQC (version 0.11.2)