FastQCFastQC Report
Fri 20 Mar 2015
L2V18DR-BC1_AACCAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL2V18DR-BC1_AACCAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87191100
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT592150.014.4389021
TTTGTGT942950.013.9666842
AACTTTG660700.013.0003762
CTTTGTG1185200.011.2863091
TTGTGTT1247100.011.0927463
TGTGTTT1344250.010.3462054
GTGTTTG1361300.010.2463375
ACTTTGT919850.09.3704163
TGTTTGA1616350.08.5958816
GAGGCTA126600.07.26792614
AGGCTAC130350.06.90261715
TGACGGG197200.06.54911210
CTACCCG143950.06.50119418
GCTACCC144650.06.500818317
GTTTGAG959900.06.33695657
GTTTGAC966700.06.2574627
CACGGTA169600.06.221677345
TTGACGG240050.06.11197579
ATGGCCC57150.06.10205345
CTCACGG182150.05.978968643
Produced by FastQC (version 0.11.2)