FastQCFastQC Report
Fri 20 Mar 2015
L2V18DR-BC12_GAGTCA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL2V18DR-BC12_GAGTCA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38748502
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT374500.018.5136471
AACTTTG407400.017.0821722
TTTGTGT930850.015.0975972
CTTTGTG947450.014.9185721
ACTTTGT666450.013.4163621
TTGTGTT1122400.013.0475573
TGTGTTT1215800.012.0952074
GTGTTTG1250700.011.6923785
CACGGTA109300.010.61930945
TGTTTGA1451600.010.0788586
CTCACGG125300.09.51512543
TCACGGT130000.09.04993144
CCTCACG134650.08.80596442
GTTTGAG710600.08.7920887
GTTTGAC617000.08.2728597
CCCTCAC144250.08.25112341
TGACGGG134200.08.03025510
AAAGTGC150050.07.902696623
TTTGAGG301150.07.76456178
TTGACGG154100.07.66617449
Produced by FastQC (version 0.11.2)