FastQCFastQC Report
Fri 20 Mar 2015
L2V18DR-BC10_GGAGAA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL2V18DR-BC10_GGAGAA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42805050
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG809250.014.0625121
TTTGTGT843450.013.4525032
TAACTTT391400.012.3548621
TTGTGTT1000050.011.7762923
AACTTTG429600.011.510962
ACTTTGT619650.011.3062251
TGTGTTT1110300.010.6778914
GTGTTTG1113700.010.5942945
TGTTTGA1283550.09.2204986
GTTTGAG605550.08.674797
CACGGTA86800.07.12670445
GTTTGAC443900.06.9664227
TTTGAGG284000.06.8942948
CTCACGG91200.06.70920643
GTTTGAT503350.06.3001247
TTTGAGT262500.06.0872058
CCTCACG104450.05.90228842
TCACGGT104600.05.89261244
TTTGAGC198750.05.57117568
CCCTCAC112550.05.53749141