FastQCFastQC Report
Fri 20 Mar 2015
L2V11DR-BC7_AAGCCT_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL2V11DR-BC7_AAGCCT_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41573796
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG739500.014.4108331
TTTGTGT812200.013.157712
TTGTGTT966200.011.5315883
TAACTTT394700.011.0474361
ACTTTGT566900.010.7135771
GTGTTTG1067050.010.4116825
TGTGTTT1084350.010.356074
AACTTTG452950.09.73457052
TGTTTGA1240150.08.9152296
GTTTGAG593750.08.6459977
TTTGAGG284950.07.21862948
GTTTGAT537800.06.39015877
GTTTGAC359250.06.3648827
TTTGAGT267100.06.20970258
TTGAGGG203900.05.264019