FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_slow_93v6_t230.3410000000257f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_slow_93v6_t230.3410000000257f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6153653
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGATCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC1145741.86188593994494No Hit
ACTTGATGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT959231.5587976767620793No Hit
ACTTGATGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC898621.4603033352709358No Hit
ACTTGATCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCG713551.1595551455371305No Hit
ACTTGATGCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACC513270.8340899299976778No Hit
ACTTGATGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCC493800.8024501869052415No Hit
ACTTGATCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT474240.7706641892222392No Hit
ACTTGATGGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGA449700.7307854375279205No Hit
ACTTGATCCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATG418780.6805388604134812No Hit
ACTTGATCGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCC383290.6228658002003038No Hit
ACTTGATGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTC362850.5896497576317676No Hit
ACTTGATCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTA345680.5617476318537948No Hit
ACTTGATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG328420.533699251485256No Hit
ACTTGATGCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAA319650.5194475541601062No Hit
ACTTGATCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC315400.5125410873833802No Hit
ACTTGATCGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGG314450.5109972889274063No Hit
ACTTGATGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGT307570.4998169380041416No Hit
ACTTGATGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAAT290150.47150854947459664No Hit
ACTTGATCGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG282390.45889815366579817No Hit
ACTTGATCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCA264630.4300372477941151No Hit
ACTTGATGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACT252400.4101628739872073No Hit
ACTTGATGGTCCTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATT242800.3945623843268381No Hit
ACTTGATGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC242060.39335984658218465No Hit
ACTTGATCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGG226900.36872407332685153No Hit
ACTTGATCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC222610.36175260450987407No Hit
ACTTGATCCCTGAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTT219170.35616242904824175No Hit
ACTTGATCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAA214210.34810217605705096No Hit
ACTTGATCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAAC211990.3444945628230906No Hit
ACTTGATCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC205730.33432174352372485No Hit
ACTTGATGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTAT184080.2991393892375797No Hit
ACTTGATGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTA183850.29876562750613334No Hit
ACTTGATCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGC178300.2897465944212324No Hit
ACTTGATGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAA171280.2783387363570874No Hit
ACTTGATCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGG169930.276144917498598No Hit
ACTTGATGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAG169180.27492612924388166No Hit
ACTTGATCGATGGTCCTAGAAACCAACAAAATAGAACCAAACGTCCTATTC168420.2736910904791024No Hit
ACTTGATGCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCAT163980.2664758640111817No Hit
ACTTGATCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATCA157720.25630304471181586No Hit
ACTTGATGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATAG155050.2519641585250257No Hit
ACTTGATCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGT154420.250940376391064No Hit
ACTTGATCCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTC152320.2475277692778582No Hit
ACTTGATGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTC151760.24661774071433668No Hit
ACTTGATCCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTG145540.23650992345522248No Hit
ACTTGATGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTT143990.23399109439547536No Hit
ACTTGATGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAA140350.22807590873258535No Hit
ACTTGATCCCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAAT139630.22690587200805765No Hit
ACTTGATACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTT138720.22542707559233519No Hit
ACTTGATCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGT133720.21730182056089284No Hit
ACTTGATGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAA130210.21159789152882033No Hit
ACTTGATCGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCATCTT128750.2092253170596392No Hit
ACTTGATAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT127440.2070965002414013No Hit
ACTTGATGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATT127180.20667398697976633No Hit
ACTTGATGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAA126750.20597521504706226No Hit
ACTTGATGCCCTCCAATTGTTCCTCGTTAAGGTATTTACATTGTACTCATT126060.20485392985272327No Hit
ACTTGATGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA119050.19346232229864116No Hit
ACTTGATCTCGTTAAGGTATTTACATTGTACTCATTCCAATTACAAGACCC118380.19237353812442787No Hit
ACTTGATGTCCTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATTA116570.18943219580304577No Hit
ACTTGATCCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCAT115190.18718962541436768No Hit
ACTTGATCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAA112940.18353326065021866No Hit
ACTTGATCCCTCCAATTGTTCCTCGTTAAGGTATTTACATTGTACTCATTC112180.18229822188543943No Hit
ACTTGATCTCTGACAATTGAATACTGATGCCCCCGACCGTCCCTATTAATC110100.17891811579235942No Hit
ACTTGATGTCGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCATC109690.17825184487978118No Hit
ACTTGATCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTT109630.17815434181940387No Hit
ACTTGATCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGA109310.17763432549739155No Hit
ACTTGATCCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGA108310.1760092744911031No Hit
ACTTGATGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTT104360.16959032301626367No Hit
ACTTGATCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGAC103980.16897280363387404No Hit
ACTTGATGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATA102100.16591770774205175No Hit
ACTTGATATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCC101520.16497517815840446No Hit
ACTTGATCCTGAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTG98700.160392534320671No Hit
ACTTGATCTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGA98480.16003502309928755No Hit
ACTTGATGCCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTT97480.15840997209299906No Hit
ACTTGATAGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGT97340.15818246495211868No Hit
ACTTGATCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGAT96410.15667116751627042No Hit
ACTTGATCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCC96150.15624865425463544No Hit
ACTTGATCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTA96030.15605364813388078No Hit
ACTTGATATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAA95730.15556613283199427No Hit
ACTTGATGACGGTATCTGATCATCTTCGATCCCCTAACTTTCGTTCTTGAT94970.15433109406721504No Hit
ACTTGATCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACG94160.1530148027521214No Hit
ACTTGATAGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTA92890.15095098797413503No Hit
ACTTGATCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC91180.14817215075338178No Hit
ACTTGATGTCACTACCTCCCTGAATTAGGATTGGGTAATTTGCGCGCCTGC90140.14648209770684176No Hit
ACTTGATACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC89770.14588082883451506No Hit
ACTTGATGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGAT88480.14378451303640294No Hit
ACTTGATGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGA88320.14352450487539678No Hit
ACTTGATCCCAGCACGACGGAGTTTCACAAGATTACCAAGACCTCTCGGCC85460.13887685899741178No Hit
ACTTGATGGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT82030.13330293404584237No Hit
ACTTGATGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTT81670.13271791568357852No Hit
ACTTGATGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCG79820.12971157132194489No Hit
ACTTGATGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCATCTTC79120.12857403561754294No Hit
ACTTGATGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGG78510.12758275450370699No Hit
ACTTGATACCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCAT76640.12454390912194757No Hit
ACTTGATAGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC74530.12111505149867892No Hit
ACTTGATCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGA73810.11994501477415123No Hit
ACTTGATGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCG73500.1194412489622018No Hit
ACTTGATCTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACT71700.11651615715088258No Hit
ACTTGATGGGCCCTGTATCGTTATTTATTGTCACTACCTCCCTGAATTAGG71620.11638615307037949No Hit
ACTTGATTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATAC71390.11601239133893315No Hit
ACTTGATCTCCGGAATCGAACCCTTATTCCCCGTTACCCGTTGAAACCATG70340.11430608778233027No Hit
ACTTGATACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGA70160.11401357860119835No Hit
ACTTGATCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCC68950.1120472668835893No Hit
ACTTGATCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTC68570.11142974750119969No Hit
ACTTGATCCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAA68250.11090973117918738No Hit
ACTTGATCTCCAATTGTTCCTCGTTAAGGTATTTACATTGTACTCATTCCA67510.10970719343453392No Hit
ACTTGATCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAG67430.10957718935403085No Hit
ACTTGATGTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAAT66790.10853715671000623No Hit
ACTTGATCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCA66170.1075296250861074No Hit
ACTTGATGATCCCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCA65900.10709086131440951No Hit
ACTTGATGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATCA65380.10624583479113951No Hit
ACTTGATAGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG65210.10596957612007048No Hit
ACTTGATCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC64790.10528705469742931No Hit
ACTTGATGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGAC63800.10367825420120375No Hit
ACTTGATGTCTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAA63120.10257321951692758No Hit
ACTTGATGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATG62690.10187444758422354No Hit
ACTTGATACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCT61770.10037940065843816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGAT5946550.044.9637371
CTTGATC2338600.044.8429262
TTGATCC1110850.044.82843
TTGATGC1082100.044.822183
GATCCCG217400.044.8180475
GATGCGC206950.044.798825
CTTGATG2567000.044.7915422
GATCGCG133200.044.780955
TGATCGC285900.044.7640274
TGATGCG336450.044.7235874
ATGCGCC123150.044.6340266
ATGCCCG35550.044.6014336
TGATCGG151250.044.5679244
GATCGCC116700.044.5595665
TGATCCC787900.044.5189934
GATCGGG62050.044.4761055
ATCGGGC51750.044.45956
ATCGCGG46300.044.446696
GATCGGC63350.044.4155125
TTGATGG789800.044.380573
Produced by FastQC (version 0.11.2)