FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_slow_93v6_t130.34100000002562.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_slow_93v6_t130.34100000002562.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6008957
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCCTCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCG742381.235455670593083No Hit
GTGGCCTGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC658811.0963799541251502No Hit
GTGGCCTCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC646901.076559542696012No Hit
GTGGCCTGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT488230.81250373400908No Hit
GTGGCCTCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT472790.786808758990953No Hit
GTGGCCTGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCC408170.6792692974837397No Hit
GTGGCCTGCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACC309450.5149812188704296No Hit
GTGGCCTCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAAC292900.4874390014772946No Hit
GTGGCCTCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC291520.4851424298759336No Hit
GTGGCCTCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGG279060.4644067181708906No Hit
GTGGCCTCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTA267950.4459176525976139No Hit
GTGGCCTCGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCC252700.4205388722202538No Hit
GTGGCCTGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGT252510.4202226775794867No Hit
GTGGCCTCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC249580.415346623382394No Hit
GTGGCCTCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGC212190.3531228464440667No Hit
GTGGCCTCGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGG209920.3493451525780597No Hit
GTGGCCTCGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG209570.3487626887661203No Hit
GTGGCCTCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCA201540.3353993047379104No Hit
GTGGCCTCCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATG198830.33088937065117957No Hit
GTGGCCTACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC191730.31907367618040866No Hit
GTGGCCTCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAA187640.3122671704923167No Hit
GTGGCCTCCCTGAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTT180940.3011171489494766No Hit
GTGGCCTCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGG175380.2918642952512391No Hit
GTGGCCTCCTGAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTG173810.2892515290091109No Hit
GTGGCCTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC172740.28747085392689614No Hit
GTGGCCTCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGA168790.28089733376357995No Hit
GTGGCCTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACT167330.2784676275766327No Hit
GTGGCCTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC146560.24390256079382827No Hit
GTGGCCTGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTC143960.23957568676227836No Hit
GTGGCCTCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGT142220.2366800095257796No Hit
GTGGCCTCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCT137360.22859208345142093No Hit
GTGGCCTGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAAT134250.22341647643675933No Hit
GTGGCCTGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTC131330.21855706406286482No Hit
GTGGCCTGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAA129500.21551161041758163No Hit
GTGGCCTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGT126850.2111015272700404No Hit
GTGGCCTGCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAA125950.20960376318219617No Hit
GTGGCCTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCG120040.1997684456720193No Hit
GTGGCCTCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTT117120.19490903329812478No Hit
GTGGCCTCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGT116700.1942100767237975No Hit
GTGGCCTCTCGTTAAGGTATTTACATTGTACTCATTCCAATTACAAGACCC115620.19241275981838446No Hit
GTGGCCTGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATT113530.18893461876994627No Hit
GTGGCCTGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTA113310.18856849865958436No Hit
GTGGCCTCGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCATCTT105900.17623690766966713No Hit
GTGGCCTCTCCAATTGTTCCTCGTTAAGGTATTTACATTGTACTCATTCCA104690.1742232470626766No Hit
GTGGCCTGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATAG103510.17225951192528088No Hit
GTGGCCTGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTAT102780.17104465883180725No Hit
GTGGCCTAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT101190.1683986089432825No Hit
GTGGCCTCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGAC99280.16522002071241315No Hit
GTGGCCTGTCCTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATTA99050.16483725877885297No Hit
GTGGCCTCTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATTATTC97450.16217456706712996No Hit
GTGGCCTGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAG95930.1596450099409931No Hit
GTGGCCTCCCAGCACGACGGAGTTTCACAAGATTACCAAGACCTCTCGGCC94730.15764799115720082No Hit
GTGGCCTGCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCAT94470.15721530375404583No Hit
GTGGCCTCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTC92870.15455261204232282No Hit
GTGGCCTCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTA92300.1536040281200215No Hit
GTGGCCTGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATA91750.1526887278441167No Hit
GTGGCCTGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATT90880.15124088922586731No Hit
GTGGCCTCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAG90430.15049200718194522No Hit
GTGGCCTGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA89770.14939364685085949No Hit
GTGGCCTCGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCT87380.14541625110647322No Hit
GTGGCCTCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCC83950.139708105749467No Hit
GTGGCCTGTCACTACCTCCCTGAATTAGGATTGGGTAATTTGCGCGCCTGC82160.13672921939697688No Hit
GTGGCCTCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCC81570.13574735182827902No Hit
GTGGCCTGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGG81050.13488197702196902No Hit
GTGGCCTGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAA80930.1346822751435898No Hit
GTGGCCTCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAAC78400.13047189387442779No Hit
GTGGCCTGTCGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCATC78300.1303054756424451No Hit
GTGGCCTGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGAT77030.12819196409626496No Hit
GTGGCCTCTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACT76600.12747636569873938No Hit
GTGGCCTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGT76530.12735987293635154No Hit
GTGGCCTCTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGA76070.12659434906923114No Hit
GTGGCCTCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGA75160.12507994315818868No Hit
GTGGCCTATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAA71940.11972127608834611No Hit
GTGGCCTCCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTG71600.11915545409960497No Hit
GTGGCCTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTC70160.11675903155905426No Hit
GTGGCCTGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAA66790.11115073714123766No Hit
GTGGCCTAGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGT66420.11053498968290171No Hit
GTGGCCTGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCATCTTC65740.10940334570541943No Hit
GTGGCCTCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGAT65300.1086711054846956No Hit
GTGGCCTCTGACAATTGAATACTGATGCCCCCGACCGTCCCTATTAATCAT65120.10837155266712677No Hit
GTGGCCTGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGATA64650.10758938697680812No Hit
GTGGCCTCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTC63130.10505982985067126No Hit
GTGGCCTGGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT61960.10311273653647379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGCCT5225050.044.978031
CCTCGCG133950.044.866265
GCCTCGC248800.044.7943274
GGCCTCC863600.044.7758183
TGGCCTC2216850.044.74982
GCCTGCC431800.044.7341464
GGCCTCG547950.044.705053
TGGCCTG2062700.044.7028472
GGCCTGG568750.044.6875723
GGCCTAC165000.044.6570173
GTGTCCT659400.044.6438831
GCCTGCG232850.044.617114
TGGCCTA666350.044.5824972
GGCCTCT618250.044.547613
CCTGCCC202300.044.5390745
GGCCTGC845400.044.5345423
GCCTGGC324550.044.5129174
CCTCCCG206100.044.5036245
GGCCTGT473750.044.4904143
CCTCGGG56800.044.469025
Produced by FastQC (version 0.11.2)