FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_slow_93v6_t090.34100000002556.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_slow_93v6_t090.34100000002556.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5227373
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTTGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC497300.9513382725893101No Hit
TAGCTTTCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCG424870.8127791913835114No Hit
TAGCTTTCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC348940.6675245864414114No Hit
TAGCTTTGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT299810.5735385632515606No Hit
TAGCTTTGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCC284530.5443078196256513No Hit
TAGCTTTGCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACC191230.36582428688367946No Hit
TAGCTTTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC180040.3444177409953336No Hit
TAGCTTTCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT168850.3230111951069878No Hit
TAGCTTTCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAAC137510.26305756256536506No Hit
TAGCTTTGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGT130710.2500491164491227No Hit
TAGCTTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC128520.24585963159698No Hit
TAGCTTTACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC124360.23790152338469053No Hit
TAGCTTTCGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG121650.2327172750060116No Hit
TAGCTTTCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC109690.20983771389567954No Hit
TAGCTTTCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGA104350.1996222576808657No Hit
TAGCTTTCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC100460.19218066129966238No Hit
TAGCTTTCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAA95670.18301735881483872No Hit
TAGCTTTCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTA95250.1822138959664826No Hit
TAGCTTTCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCA94930.18160173379630648No Hit
TAGCTTTGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATT94670.18110435203303837No Hit
TAGCTTTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATAT82220.15728741759962414No Hit
TAGCTTTGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTAT81010.1549726793936457No Hit
TAGCTTTGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTC77480.1482197654538905No Hit
TAGCTTTCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGG76210.14579024684100408No Hit
TAGCTTTGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTC74170.14188771300613137No Hit
TAGCTTTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCG71830.13741127713671858No Hit
TAGCTTTGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAAT68190.13044793245096534No Hit
TAGCTTTGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTA66870.12792276349898887No Hit
TAGCTTTGCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAA62950.12042377691433154No Hit
TAGCTTTGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA62690.11992639515106344No Hit
TAGCTTTCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGACC61780.11818555897962514No Hit
TAGCTTTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACT60520.11577517043455672No Hit
TAGCTTTGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAG60090.11495257751838256No Hit
TAGCTTTCGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGG59190.11323087141476226No Hit
TAGCTTTCGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCC58870.11261870924458614No Hit
TAGCTTTCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGC58690.11227436802386209No Hit
TAGCTTTGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAA57210.10944311798679758No Hit
TAGCTTTCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTT57150.10932833757988955No Hit
TAGCTTTCTTGGTTTCTTCCAAGTATTGGACTTCAATGACTGGGTTACCAT56360.1078170622222673No Hit
TAGCTTTGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGA55060.10533015340592683No Hit
TAGCTTTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGT53100.10158066011359816No Hit
TAGCTTTGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGG52960.10131283916414613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTTT5106000.044.8905261
AGCTTTG2406250.044.8208772
AGCTTTC1657850.044.7552572
GCTTTGC882150.044.755233
GCTTTCC542300.044.3692443
GCTTTCG397900.044.3654253
CTTTGCG266750.044.3286824
CTTTCCC292600.044.2868584
GCTTTGT594950.044.212213
AGCTTTA750450.044.168992
GCTTTGG694600.044.1550063
CTTTGCC398250.044.071434
TTTCGCG58200.043.7101825
CTTTCCG110900.043.363814
CTTTGGC385350.043.176914
CTTTCGG157350.043.1709374
TTTCCCG117650.043.1693045
GCTTTCT546000.042.9933243
GCTTTAG241150.042.991153
GCTTTAC147450.042.973593
Produced by FastQC (version 0.11.2)