FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_slow_93v6_t070.34100000002549.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_slow_93v6_t070.34100000002549.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7175387
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC350000.48777856859846025No Hit
CTCAGATGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC167040.232795805996248No Hit
CTCAGATCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCG163390.22770897235229265No Hit
CTCAGATCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC129340.18025508589292813No Hit
CTCAGATGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGA125760.17526580796269248No Hit
CTCAGATCTTGTCAGTGACACCGTACTTCTTAGCGACAGTGGCAGTGTCAC116350.1621515327326596No Hit
CTCAGATGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCC113660.15840260601971715No Hit
CTCAGATGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATAT104290.14534407691180978No Hit
CTCAGATCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGACC100110.13951860714969103No Hit
CTCAGATCTTGGTTTCTTCCAAGTATTGGACTTCAATGACTGGGTTACCAT95860.13359558167385258No Hit
CTCAGATGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT94430.1316026578078646No Hit
CTCAGATGTCTTGGTTTCTTCCAAGTATTGGACTTCAATGACTGGGTTACC92420.12880141517105628No Hit
CTCAGATATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTA88650.1235473431607243No Hit
CTCAGATTAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTCCA81240.11322037403696832No Hit
CTCAGATACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGAC75280.10491420184026311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT7010200.044.9635121
TCAGATC1989700.044.7462232
TCAGATG3303950.044.71722
CAGATCG395500.044.552533
CAGATCC510250.044.2309573
CAGATGG1007000.044.1672483
TCAGATA1275250.044.1028632
AGATGCC420250.044.010354
CAGATGT981950.043.9975053
AGATCCC222500.043.9234
CAGATCT771900.043.8191833
CAGATGC942350.043.7674563
CAGATGA431900.043.635933
GATCGGC68000.043.627985
AGATGGC506250.043.6026884
AGATCGC90950.043.4264224
AGATGTC304900.043.3615074
AGATGCG214600.043.317954
CAGATAG401200.043.2804373
CAGATCA348250.043.2215233