FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_slow_93v6_t050.3410000000253c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_slow_93v6_t050.3410000000253c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5560948
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCAGTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC285040.5125744747118657No Hit
GATCAGTCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGACC89130.16027842734727965No Hit
GATCAGTCTTGGTTTCTTCCAAGTATTGGACTTCAATGACTGGGTTACCAT78730.1415765801082837No Hit
GATCAGTGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGA71770.1290607284944941No Hit
GATCAGTCTTGTCAGTGACACCGTACTTCTTAGCGACAGTGGCAGTGTCAC71140.1279278281329011No Hit
GATCAGTGTCTTGGTTTCTTCCAAGTATTGGACTTCAATGACTGGGTTACC69200.12443921432101146No Hit
GATCAGTGTTCTAACCATTTGGCTAACTTCAATGCATTTTCACCGTGTCTT69010.12409754595799133No Hit
GATCAGTCTTGGTTTCTTCCAAGTATTGGACTTCGATGACTGGGTTACCAT63970.11503434306524714No Hit
GATCAGTCGCGTTAGCCATAACAAAATCGGAAGCTTCGATAACGGCAGAAT57410.10323779326834202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAGT5391000.044.9685551
TCAGTCG335750.044.8140753
ATCAGTG2326950.044.757232
CAGTCGG117500.044.7007064
ATCAGTC1630600.044.694432
TCAGTGC636900.044.6915933
TCAGTCC343350.044.4707073
CAGTGCG132550.044.3939064
CAGTGCC267300.044.339894
CAGTCGC99550.044.28744
CAGTCCC146250.044.2649774
ATCAGTA1080950.044.2183072
CAGTGCA121750.044.174944
CAGTCCG75200.044.0306744
TCAGTGT733550.043.9947243
AGTCCCG53350.043.806515
TCAGTCT658050.043.7781833
GTGCCCG18450.043.764746
AGTCGCG27400.043.673565
AGTGCCG68050.043.4996035
Produced by FastQC (version 0.11.2)