FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_slow_93v6_t030.3410000000252f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_slow_93v6_t030.3410000000252f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5967585
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC227110.3805727107364202No Hit
ACAGTGTCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGACC80480.1348619248825111No Hit
ACAGTGTGTGACCGTTTTCTCTCAAGACGTCGTATTGAGCCAAGAACATAC74360.12460652005794638No Hit
ACAGTGTGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGA70480.11810472745675178No Hit
ACAGTGTGTCTTGGTTTCTTCCAAGTATTGGACTTCAATGACTGGGTTACC62500.10473248391099582No Hit
ACAGTGTGTCTTGGTTTCTTCCAAGTATTGGACTTCGATGACTGGGTTACC61460.10298973537871685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGT5276400.044.9444241
CAGTGTG2335650.044.8197942
AGTGTGC684100.044.6148573
CAGTGTA1062850.044.4319272
AGTGTGT691000.044.4102363
CAGTGTC1462400.044.395772
AGTGTGG653050.044.3905333
AGTGTCC319850.044.1073533
AGTGTCG314900.044.0149573
AGTGTCT547850.043.902493
GTCCGGC15750.043.554616
AGTGTGA334300.043.4523123
ACATTGT629800.043.150061
AGTGTAG338550.043.0064963
TGTGCGG90900.042.9325685
GTGTGCG178750.042.6332634
CAGTGTT480950.042.615252
TGTGCCC93250.042.453665
AGTGTAT322300.042.4530373
GTGCGGC47950.042.3090026
Produced by FastQC (version 0.11.2)