FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_slow_93v6_t020.34100000002512.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_slow_93v6_t020.34100000002512.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6773624
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACCATCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC169200.24979243016736682No Hit
TGACCATGTGACCGTTTTCTCTCAAGACGTCGTATTGAGCCAAGAACATAC103270.15245900864884143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCATG3287000.044.8465162
ACCATGC972700.044.677883
TGACCAT6702300.044.4009671
CCATGCG252150.044.325324
ACCATGG922400.044.2904553
GACCATA1191900.044.2683142
ACCATGT1017150.044.0898323
CATGCCC105100.043.701355
CCATGCA190600.043.6401144
CCATGCC367050.043.502464
ACCATGA425000.043.4188773
CCATGCT185200.043.3336944
CCATGGC427550.043.3282664
ACCATCT659550.043.2188843
ACCATCC365500.043.151553
CCATGTG267200.043.0829774
CATGCGG115350.043.0549745
CCATCGG151200.042.9920234
ACCATCG339900.042.9869583
CATGCCG95450.042.8881535
Produced by FastQC (version 0.11.2)