FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_slow_93v6_t010.34100000002505.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_slow_93v6_t010.34100000002505.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6582055
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGTTGTGCCTTTTGGTAACGACTGATCGATAGATACGACCTTTAAAAC73450.11159128873885131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTT6364700.044.9308241
GATGTTG3781550.044.6279452
ATGTTGC1391200.044.5579953
GATGTTC1538850.044.511252
ATGTTGG1273900.044.075523
TGTTGCC560350.044.073314
ATGTTCG393100.044.0693323
ATGTTCC432100.043.8448453
GATGTTA791600.043.8056452
GTTGCCC148700.043.7441565
TGTTGCG398750.043.7040444
GTTGCGG190900.043.605965
GTTGCCG149000.043.3692635
TGTTGGC667000.043.1264274
TGTTCGG209300.043.039384
GAGATCG99850.043.02275513
ATGTTGT884900.042.9869653
GCACACG98950.042.9039925
CACACGT99350.042.73125526
AGATCGG100850.042.7286614
Produced by FastQC (version 0.11.2)