FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_med_92v2_t200.3410000000267e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_med_92v2_t200.3410000000267e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8845449
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGCTGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC1195311.3513276714387252No Hit
AAATGCTCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC791750.8950930585886595No Hit
AAATGCTCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT746350.8437672299054576No Hit
AAATGCTCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCG720500.8145431622521366No Hit
AAATGCTGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT623960.7054022921843763No Hit
AAATGCTCGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCC616840.6973529551750285No Hit
AAATGCTACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC586370.6629058626645182No Hit
AAATGCTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGT564210.6378534317477835No Hit
AAATGCTCGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCT539790.6102460146454973No Hit
AAATGCTGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCC533430.6030558765304056No Hit
AAATGCTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCG514800.581994198372519No Hit
AAATGCTCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTA506490.5725995367787435No Hit
AAATGCTCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC462860.52327473709927No Hit
AAATGCTGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTC453190.51234256169472No Hit
AAATGCTCGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG379560.4291020161893421No Hit
AAATGCTCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAAC379360.42887591121716945No Hit
AAATGCTGCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACC377500.42677313497596336No Hit
AAATGCTGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTC367350.41529830763819897No Hit
AAATGCTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC363240.4106518504600501No Hit
AAATGCTCGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGG340170.3845706419199297No Hit
AAATGCTGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAAT325700.36821194718323513No Hit
AAATGCTCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC324840.36723969580289256No Hit
AAATGCTGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGT302370.34183680217929013No Hit
AAATGCTGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTAT302220.34166722345016065No Hit
AAATGCTATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAA301270.34059322483234034No Hit
AAATGCTCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGC292770.33098376351500075No Hit
AAATGCTCCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATG287310.32481109777468614No Hit
AAATGCTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTC286790.3242232248470372No Hit
AAATGCTCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAA270480.3057843643663538No Hit
AAATGCTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACT266950.30179361160750573No Hit
AAATGCTCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGG263170.29752022763344177No Hit
AAATGCTGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGA247340.2796240190859729No Hit
AAATGCTCCTGAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTG239170.2703876309727183No Hit
AAATGCTGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACT222600.25165483402821043No Hit
AAATGCTGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATT220280.24903201635100716No Hit
AAATGCTGCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAA214410.24239583541773851No Hit
AAATGCTCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCA214230.24219234094278314No Hit
AAATGCTCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGAC209480.23682234785368161No Hit
AAATGCTGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAA203200.22972265172745893No Hit
AAATGCTGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATT200260.22639890863652032No Hit
AAATGCTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC199800.22587886720052314No Hit
AAATGCTCCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTC199310.22532491001870003No Hit
AAATGCTGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAA198630.22455615311331284No Hit
AAATGCTCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACG194010.21933312825612358No Hit
AAATGCTCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTA189690.21444926085719332No Hit
AAATGCTCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGA187480.21195080091468504No Hit
AAATGCTCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG186790.21117073876068923No Hit
AAATGCTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCC186300.21061678157886615No Hit
AAATGCTCCCTGAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTT184330.2083896476029651No Hit
AAATGCTGTCCTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATTA181370.20504329401480917No Hit
AAATGCTCCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAA179260.20265788655838726No Hit
AAATGCTCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGG179160.20254483407230092No Hit
AAATGCTCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCT177910.20113167799622156No Hit
AAATGCTCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGT177720.2009168782726575No Hit
AAATGCTGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA173880.19657566280694175No Hit
AAATGCTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG171300.19365890866591395No Hit
AAATGCTGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTT170410.19265274153974546No Hit
AAATGCTCGATGGTCCTAGAAACCAACAAAATAGAACCAAACGTCCTATTC170340.19257360479948502No Hit
AAATGCTCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGC169780.19194051087740147No Hit
AAATGCTGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTA168080.19001861861393357No Hit
AAATGCTCTCCAATTGTTCCTCGTTAAGGTATTTACATTGTACTCATTCCA166190.1878819266269016No Hit
AAATGCTGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTT163700.18506691972335151No Hit
AAATGCTGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATAG160820.1818110081240647No Hit
AAATGCTCCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTG159720.18056743077711487No Hit
AAATGCTCTCGTTAAGGTATTTACATTGTACTCATTCCAATTACAAGACCC157540.1781028865804325No Hit
AAATGCTGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTT154910.17512960619636156No Hit
AAATGCTCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTC153720.173784281611934No Hit
AAATGCTGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAA149940.16951089763787003No Hit
AAATGCTCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCC148700.16810904681039934No Hit
AAATGCTCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAA143080.16175549709234657No Hit
AAATGCTCGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCATCTT141980.16051191974539675No Hit
AAATGCTCTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACT141830.16034234101626724No Hit
AAATGCTATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTT140190.15848828024445114No Hit
AAATGCTGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGG139910.15817173328340933No Hit
AAATGCTGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAG139300.15748211311828264No Hit
AAATGCTCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTT139300.15748211311828264No Hit
AAATGCTCTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATTATTC138460.15653247223515732No Hit
AAATGCTAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT138340.15639680925185367No Hit
AAATGCTCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAG137680.15565066284368378No Hit
AAATGCTCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGT136890.15475754820360163No Hit
AAATGCTCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCC135840.15357049709969497No Hit
AAATGCTAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCG134090.1515920785931839No Hit
AAATGCTGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGAT133790.15125292113492486No Hit
AAATGCTGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAA132300.14956843909223827No Hit
AAATGCTCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGT131460.14861879820911295No Hit
AAATGCTGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATG131000.14809875677311576No Hit
AAATGCTAGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGT128560.14534027611260886No Hit
AAATGCTCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGA127780.14445846672113535No Hit
AAATGCTGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATA127050.14363318357270502No Hit
AAATGCTCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAAC120520.13625085623126648No Hit
AAATGCTCCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGA115830.13094869463381678No Hit
AAATGCTGTCGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCATC115740.13084694739633906No Hit
AAATGCTCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGAT114640.12960337004938924No Hit
AAATGCTCTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGA114180.12908332861339203No Hit
AAATGCTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCT110410.12482124988793672No Hit
AAATGCTCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCA109860.12419946121446182No Hit
AAATGCTGCCCTGTATCGTTATTTATTGTCACTACCTCCCTGAATTAGGAT109000.12322720983411921No Hit
AAATGCTCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTC107430.12145228580256355No Hit
AAATGCTGCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCAT105480.11924776232387978No Hit
AAATGCTCTCTGACAATTGAATACTGATGCCCCCGACCGTCCCTATTAATC104880.1185694474073617No Hit
AAATGCTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGT104350.11797026923110404No Hit
AAATGCTGTCACTACCTCCCTGAATTAGGATTGGGTAATTTGCGCGCCTGC103180.11664755514389376No Hit
AAATGCTCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTGA103000.11644406066893835No Hit
AAATGCTGCCCTCCAATTGTTCCTCGTTAAGGTATTTACATTGTACTCATT99080.11201240321435352No Hit
AAATGCTGGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT97920.11070099437575187No Hit
AAATGCTGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCATCTTC97100.10977396398984382No Hit
AAATGCTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG96070.10860952338315444No Hit
AAATGCTGGGCCCTGTATCGTTATTTATTGTCACTACCTCCCTGAATTAGG96070.10860952338315444No Hit
AAATGCTTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATAC95480.10794251371524498No Hit
AAATGCTTGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT92520.1045961601270891No Hit
AAATGCTCCCTCCAATTGTTCCTCGTTAAGGTATTTACATTGTACTCATTC91920.103917845210571No Hit
AAATGCTCCCAGCACGACGGAGTTTCACAAGATTACCAAGACCTCTCGGCC91600.10355607725509469No Hit
AAATGCTGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGATA91170.10306995156492338No Hit
AAATGCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGG90990.10286645708996796No Hit
AAATGCTCGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG90940.1028099308469248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCTG3359800.044.9088362
ATGCTCG964900.044.8925743
GCTCCCG264450.044.8312035
ATGCTGC1421250.044.7974933
AAATGCT8648900.044.762141
ATGCTGT846950.044.7282143
ATGCTCC1297100.044.6528743
TGCTCGG202200.044.618224
GCTGCCC287450.044.6087075
CTCGCGG66450.044.607936
GCTGCGC297800.044.6066555
TGCTCCC787000.044.5994764
TGCTCGC441500.044.598124
GCTCGGC83200.044.5517545
ATGCTCA338050.044.5067563
ATGCTAC273050.044.49983
ATGCTGG779650.044.4982573
CTCCCGT147250.044.460936
GCTCGCC157750.044.4424865
CTCCCGG50650.044.3585856
Produced by FastQC (version 0.11.2)