FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_med_92v2_t160.341000000025e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_med_92v2_t160.341000000025e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7076807
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACGCTGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC911301.287727643271888No Hit
TTACGCTCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCG694030.9807106510040474No Hit
TTACGCTCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC666910.9423882833034729No Hit
TTACGCTCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT570650.8063664870329232No Hit
TTACGCTGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT479620.6777350293712969No Hit
TTACGCTCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTA445730.629846200412135No Hit
TTACGCTGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCC437860.6187253658323592No Hit
TTACGCTACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC416650.5887542220665337No Hit
TTACGCTCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAAC392720.5549395369974058No Hit
TTACGCTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCG366110.5173378332912004No Hit
TTACGCTGCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACC364520.5150910573087553No Hit
TTACGCTCGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCC346520.4896558575074889No Hit
TTACGCTCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC321240.45393353245326595No Hit
TTACGCTCGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCT306430.4330060152834463No Hit
TTACGCTGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTC301490.4260254660046543No Hit
TTACGCTCGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG299440.42312867936062126No Hit
TTACGCTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGT284850.4025120368550393No Hit
TTACGCTCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGG269650.3810334236895255No Hit
TTACGCTCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGC263720.3726539384216639No Hit
TTACGCTATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAA251960.3560362745515033No Hit
TTACGCTCGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGG251500.35538626388991534No Hit
TTACGCTCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAA235100.33221197073765046No Hit
TTACGCTGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAAT226930.3206672161611868No Hit
TTACGCTCCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATG225390.31849109351152294No Hit
TTACGCTGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTC221170.3125279522247816No Hit
TTACGCTGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGT217630.3075256962638659No Hit
TTACGCTGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTAT216770.3063104589400276No Hit
TTACGCTCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCA208510.2946385283645576No Hit
TTACGCTCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC206450.29172761105396827No Hit
TTACGCTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC205840.29086564039403645No Hit
TTACGCTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACT192330.2717751098765305No Hit
TTACGCTGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATT183330.2590575099758973No Hit
TTACGCTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC169330.23927457679713465No Hit
TTACGCTCCTGAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTG167090.23610930748853262No Hit
TTACGCTGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAA163780.23143205685841084No Hit
TTACGCTCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGA154480.21829053696108994No Hit
TTACGCTCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGG152520.21552092631606318No Hit
TTACGCTGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGA151980.21475787032202517No Hit
TTACGCTCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTA151910.21465895565613136No Hit
TTACGCTGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAA149130.21073063035349135No Hit
TTACGCTCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTT146630.20719796371442656No Hit
TTACGCTGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGA142760.20172939575715432No Hit
TTACGCTCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCT142490.20134786776013536No Hit
TTACGCTGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTA142180.2009098170968913No Hit
TTACGCTCCCTGAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTT140750.19888913177934625No Hit
TTACGCTGCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAA138440.19562494780485043No Hit
TTACGCTCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGT137550.19436731848134337No Hit
TTACGCTGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATAG137070.19368904648664292No Hit
TTACGCTGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATT134700.1903400785128095No Hit
TTACGCTCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGAC133860.18915310252208378No Hit
TTACGCTCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCC130130.18388236389659912No Hit
TTACGCTCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCC126510.17876706260323336No Hit
TTACGCTCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTC125690.1776083479456201No Hit
TTACGCTCTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGA124070.17531917996350613No Hit
TTACGCTCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGT121940.17230934798702296No Hit
TTACGCTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTC117880.16657229736518178No Hit
TTACGCTGTCCTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATTA117180.16558315070624366No Hit
TTACGCTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCC115950.1638450787198238No Hit
TTACGCTCCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTG115930.16381681738671128No Hit
TTACGCTGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGAT114230.16141460407214722No Hit
TTACGCTGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGG114060.16117438274069082No Hit
TTACGCTGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAG113180.15993088408374No Hit
TTACGCTCGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCATCTT112980.15964827075261484No Hit
TTACGCTGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTT110380.1559742974479875No Hit
TTACGCTCTCCAATTGTTCCTCGTTAAGGTATTTACATTGTACTCATTCCA109980.15540907078573712No Hit
TTACGCTGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTT109510.15474492945759294No Hit
TTACGCTCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAG109400.1545894921254741No Hit
TTACGCTCTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATTATTC107150.1514100921503158No Hit
TTACGCTCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAA105020.14840026017383265No Hit
TTACGCTGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAA104200.14724154551621937No Hit
TTACGCTCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGT104180.14721328418310686No Hit
TTACGCTCTCGTTAAGGTATTTACATTGTACTCATTCCAATTACAAGACCC104110.14711436951721307No Hit
TTACGCTGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACT103640.1464502281890689No Hit
TTACGCTGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATG102250.14448606553774887No Hit
TTACGCTAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCG101950.14406214554106112No Hit
TTACGCTCTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACT100290.1417164548927221No Hit
TTACGCTCGATGGTCCTAGAAACCAACAAAATAGAACCAAACGTCCTATTC98350.13897510558080783No Hit
TTACGCTCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGAT98290.13889032158147027No Hit
TTACGCTCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACG97990.13846640158478252No Hit
TTACGCTGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATA96270.13603592693710595No Hit
TTACGCTCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTC95280.1346369909480363No Hit
TTACGCTCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGA94910.13411415628545473No Hit
TTACGCTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGT93000.13141519897320925No Hit
TTACGCTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG90160.12740208967123168No Hit
TTACGCTCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAAC90030.12721839100600033No Hit
TTACGCTGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTT88560.1251411830222302No Hit
TTACGCTGCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCAT86810.12266831637488487No Hit
TTACGCTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCT84750.11975739906429553No Hit
TTACGCTATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTT84210.11899434307025754No Hit
TTACGCTGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGATA84160.11892368973747623No Hit
TTACGCTCCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTC83960.11864107640635105No Hit
TTACGCTAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT83610.11814650307688199No Hit
TTACGCTAGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGT81270.11483992710271736No Hit
TTACGCTGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAA80810.11418991644112945No Hit
TTACGCTGTCGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCATC80210.1133420764477539No Hit
TTACGCTCGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTG77600.10965397247657029No Hit
TTACGCTCTGACAATTGAATACTGATGCCCCCGACCGTCCCTATTAATCAT77070.10890504714908857No Hit
TTACGCTGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCATCTTC75780.10708219116333113No Hit
TTACGCTCCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGA74430.10517455117823617No Hit
TTACGCTGCCCTGTATCGTTATTTATTGTCACTACCTCCCTGAATTAGGAT72140.10193862853685284No Hit
TTACGCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGG72060.10182558320440277No Hit
TTACGCTGGGCCCTGTATCGTTATTTATTGTCACTACCTCCCTGAATTAGG71010.10034186321599557No Hit
TTACGCTGGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT70970.10028534054977055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCT6937850.045.0066451
TACGCTG2718850.044.957462
ACGCTGC1144250.044.9546473
TACGCTC2886900.044.953782
ACGCTCC1062500.044.947843
CGCTCGC342150.044.9019624
ACGCTCG739700.044.8998763
CGCTCCC638900.044.899414
CGCTCGG172850.044.831274
GCTCCCG222150.044.8042225
ACGCTGT661850.044.780753
ACGCTCT799950.044.7687343
ACGCTAC210000.044.7631263
ACGCTGG669200.044.719163
CGCTGGG142750.044.6565254
CGCTGCG370250.044.610764
GCTGCCC227150.044.5903785
CTCGCGG58850.044.5622636
CGCTCTC333100.044.541744
ACGCTGA256100.044.5401153
Produced by FastQC (version 0.11.2)