FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_med_92v2_t090.341000000025dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_med_92v2_t090.341000000025dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5586524
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACAGTGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC351050.6283871688370085No Hit
TCACAGTCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCG309600.5541907633440759No Hit
TCACAGTCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC304170.5444709447234094No Hit
TCACAGTCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT181550.32497846603719954No Hit
TCACAGTGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCC180900.3238149518376722No Hit
TCACAGTGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT155570.2784736984930164No Hit
TCACAGTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC153890.27546646179269973No Hit
TCACAGTGCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACC144120.25797794836288185No Hit
TCACAGTCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC130700.2339558551972568No Hit
TCACAGTCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAAC124840.22346632718305692No Hit
TCACAGTCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAA101760.18215262299061097No Hit
TCACAGTCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTA98230.17583384587625506No Hit
TCACAGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGT95930.1717167956317739No Hit
TCACAGTACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC93840.1679756499748323No Hit
TCACAGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCG93610.16756394495038418No Hit
TCACAGTCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCA88530.15847063397561703No Hit
TCACAGTCGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTG88150.15779042567435494No Hit
TCACAGTATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAA86860.1554812974937546No Hit
TCACAGTCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC86760.15530229530921197No Hit
TCACAGTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGC82810.1482317090197769No Hit
TCACAGTCGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCT82260.14724719700479225No Hit
TCACAGTGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTC80760.14456216423665236No Hit
TCACAGTGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGT76790.13745577751030874No Hit
TCACAGTCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGG76610.13713357357813197No Hit
TCACAGTCGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGG71250.12753905648664537No Hit
TCACAGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACT69200.12386951170352083No Hit
TCACAGTGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTC67130.12016416648348777No Hit
TCACAGTCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGC65240.11678102519563148No Hit
TCACAGTGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAAT62940.1126639749511503No Hit
TCACAGTCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGA57920.10367806528710877No Hit
TCACAGTCCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATG57780.10342746222874903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACAGT5509000.044.9272081
CACAGTG2265850.044.8996282
ACAGTGC775200.044.831223
CACAGTC1915950.044.7830772
CAGTGCG198050.044.7805754
CAGTCGC167450.044.7700774
ACAGTCG441100.044.7657363
CAGTCCC295500.044.6729164
ACAGTCC550400.044.5967753
CAGTGCC334250.044.5947044
CACAGTA962200.044.5502052
CAGTCGG135550.044.4374124
AGTCCCG104500.044.4249765
AGTCCCC93400.044.3825535
CAGTCCG88600.044.3757254
ACAGTGT643600.044.3594323
AGTCGCG63950.044.3515055
CAGTGCA132900.044.2403264
AGTGCGC93550.044.239265
GTGCGCC53100.044.050746
Produced by FastQC (version 0.11.2)