FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_med_92v2_t050.341000000025cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_med_92v2_t050.341000000025cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18186473
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAACTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC430150.23652194683378133No Hit
TGAAACTCTTGTCAGTGACACCGTACTTCTTAGCGACAGTGGCAGTGTCAC188510.10365396303065472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAACT17959650.044.8926961
GAAACTC5903250.044.799862
GAAACTG7683500.044.6788332
AAACTGC2397700.044.5047383
GAAACTA3117850.044.4908682
AAACTCG1495650.044.4000323
ACTCGCG115900.044.3631635
ACTCCCG173900.044.3245355
AACTCGC448100.044.291854
AACTGCC1028100.044.100694
ACTCGGC271150.044.0800065
AAACTCC1385000.044.0106283
AAACTGT2112200.043.9840433
AACTGTC699850.043.9239654
AAACTGG2424250.043.910633
AACTCCG393000.043.8914034
AAACTAG1072150.043.850083
AACTCCC520550.043.791984
AAACTCT2095800.043.759423
AACTGCG541150.043.750094
Produced by FastQC (version 0.11.2)