FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_med_92v2_t035.341000000025b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_med_92v2_t035.341000000025b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9760278
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAGTTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC167020.1711221750036218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAGTT9621800.044.881821
AAGTTGC1308800.044.5323873
CAAGTTC3019400.044.446312
CAAGTTG4307700.044.423442
AAGTTCG721650.044.2096983
CAAGTTA1616650.044.0880662
AAGTTCC667850.044.016093
AGTTGCC515650.043.957394
AGTTCGC211250.043.928374
AGTTCGG289650.043.802124
AGTTGCG321800.043.745314
AAGTTGT1255100.043.583083
AGTTCCC239150.043.5434884
AAGTTGG1290950.043.43033
AAGTTCT1124800.043.1793
AGTTCCG196500.043.1281624
GTTGCCC137350.043.0988165
AGTTGGC645400.043.0169454
GTTCGCG56450.042.7507635
CAAGTTT826900.042.6081052
Produced by FastQC (version 0.11.2)