FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_med_92v2_t025.341000000025a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_med_92v2_t025.341000000025a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7992182
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAAATCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC156560.1958914349047607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAAAT7847450.044.8688351
CGAAATG3217600.044.8577422
CGAAATC2804550.044.6310772
CGAAATA1340650.044.4561922
GAAATGC983000.044.4211853
GAAATCC663350.044.016593
AAATGCC442650.043.989854
CGAAATT527050.043.838352
GAAATGT956500.043.7282563
GAAATGG1011000.043.584723
GAAATCG628200.043.5254973
GAAATCT1100750.043.43543
AATCGGG57900.042.73215
AATGCCC135550.042.6787385
AAATCGC191900.042.6650244
AAATCCC260300.042.523084
AATCCCG84800.042.4919625
AATCGGC102450.042.43855
AAATCCG177600.042.313514
AAATGCG188850.042.2106744
Produced by FastQC (version 0.11.2)