FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_med_92v2_t014.34100000002599.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_med_92v2_t014.34100000002599.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9358275
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACTT9274650.044.8024371
GTACTTC2846650.044.496962
TACTTGC1327000.044.479253
GTACTTG4077050.044.4034772
TACTTCG697700.044.386433
GTACTTA1558900.044.2704052
ACTTGCC493600.043.9418264
TACTTCC637750.043.843343
TACTTGG1120650.043.7777673
TACTTAG525250.043.761323
TACTTCT1057650.043.5548023
ACTTCCG203050.043.445594
TACTTGT1225850.043.4374163
ACTTGCG357050.043.4104544
ACTTCGG261750.043.2151534
TACTTAT427700.043.1348763
ACTTCCC209050.042.8442154
GTACTTT910100.042.8086852
ACTTCGC229450.042.6424644
ACTTACG77850.042.5295034
Produced by FastQC (version 0.11.2)