FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_med_92v2_t007.3410000000258c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_med_92v2_t007.3410000000258c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7824244
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGTT7705150.044.893361
AACGTTG3540300.044.661512
ACGTTGC1273450.044.655733
ACGTTCG656900.044.6247333
CGTTCCG205150.044.4141354
AACGTTC2586700.044.3894962
CGTTGCC534100.044.382054
ACGTTCC689650.044.3743743
ACGTTGG1020150.044.256673
CGTTCGG243550.044.254684
CGTTGCG299800.044.2115064
ACGTTGT957600.044.0212443
CGTTCGC225950.044.0087174
ACGTTCT884850.043.8098873
CGTTCCC246200.043.778344
AACGTTA1121600.043.674752
ACGTTAG360500.043.387033
CGTTGGC555100.043.322974
GTTGCCC139650.043.260355
CGTTGGG182650.043.1124734
Produced by FastQC (version 0.11.2)