FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_fast_93v3_t160.34100000002661.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_fast_93v3_t160.34100000002661.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6853398
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC157800.2302507456884891No Hit
TCTGATTCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGACC110380.16105879156587724No Hit
TCTGATTCAACAGCGTCTTCGGTGTAACCCAAAACACCCTTCAACTTACCT104040.15180790609271488No Hit
TCTGATTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATAT100310.1463653504436777No Hit
TCTGATTCTTGTCAGTGACACCGTACTTCTTAGCGACAGTGGCAGTGTCAC75500.1101643301614761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATT6048000.044.774861
CTGATTG2642200.044.6695822
CTGATTC2092150.044.604822
CTGATTA930250.044.600962
TGATTCC542100.044.4958043
GATTGCC393500.044.4881064
TGATTGC880050.044.487023
TGATTCG575750.044.114223
GATTCCC215650.043.8380854
GATTGGC497600.043.8011054
TGATTGG945300.043.724313
GATTCGG285950.043.6953934
ATTCCGG99850.043.6782345
GATTAGC199350.043.6652954
GATTGCG216100.043.517824
ATTCGGG81050.043.513945
GATTCCG164800.043.511374
GATTCGC157850.043.4607244
ATTGCCC128000.043.43845
ATTCCCG92800.043.4093135
Produced by FastQC (version 0.11.2)