FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_fast_93v3_t090.34100000002655.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_fast_93v3_t090.34100000002655.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6677533
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCGATCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC313330.4692301782709273No Hit
TATCGATCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCG213140.3191897366886843No Hit
TATCGATCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC208880.31281013512026073No Hit
TATCGATGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTC146480.21936245017433834No Hit
TATCGATGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGAT130970.19613530925268358No Hit
TATCGATGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCC111180.16649861558153287No Hit
TATCGATCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT105740.15835189432983707No Hit
TATCGATCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGACC91640.13723631167378733No Hit
TATCGATCAACAGCGTCTTCGGTGTAACCCAAAACACCCTTCAACTTACCT81290.12173657546881461No Hit
TATCGATGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATAT73220.10965127390609676No Hit
TATCGATCTTGTCAGTGACACCGTACTTCTTAGCGACAGTGGCAGTGTCAC71940.10773439831746245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAT6570900.044.846811
TCGATGC1014700.044.8215183
ATCGATC2296100.044.7428632
CGATCGG224450.044.7372364
CGATCCG186050.044.7338874
ATCGATG2868500.044.721162
TCGATCG563700.044.716433
CGATGCC463100.044.560344
TCGATCC684050.044.4550673
CGATGCG240400.044.417014
TCGATCT746450.044.3730353
GATCGGC102750.044.2865755
CGATCGC199900.044.166344
CGATCCC301400.044.159374
CGATCTC244250.044.094474
CGATCTG211300.044.0829124
CGATCCT117700.043.9748844
GATCGCC81450.043.9652985
GATCGCG60050.043.938285
CGATGGC493000.043.9338044
Produced by FastQC (version 0.11.2)