FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_fast_93v3_t050.34100000002648.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_fast_93v3_t050.34100000002648.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7551576
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTATCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC100670.13330992100192066No Hit
AGTCTATCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGACC94490.12512619882260337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAT7442950.044.987771
GTCTATG3593750.044.8969152
CTATGCG415100.044.7942964
GTCTATC2355850.044.788812
TCTATGC1401800.044.745463
CTATGCC551700.044.6537174
GTCTATA983250.044.5682032
TCTATGG1087650.044.552333
CTATGGC599350.044.5486264
CTATCGG303300.044.4351774
TCTATCC643550.044.4218373
CTATCCG220950.044.3222664
TCTATGT849600.044.259153
CTATGGG203200.044.1759154
CTATCGC231550.044.1097154
TATGCGG203300.044.087225
CTATGTC326450.044.0813674
CTATGCA227650.044.0552144
TCTATCG698800.043.951343
TCTATCT730150.043.88483
Produced by FastQC (version 0.11.2)