FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_fast_93v3_t035.3410000000263b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_fast_93v3_t035.3410000000263b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8382703
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTATCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC143280.17092338831520096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTAT8177900.044.9464761
ACTTATG3997750.044.9114652
CTTATGC1511600.044.766073
ACTTATC2534200.044.7599642
CTTATGG1212350.044.728413
CTTATGT969800.044.5734523
ACTTATA1069350.044.5130232
TTATGCG416500.044.4675834
TTATGCC624400.044.4276664
CTTATCC700400.044.254083
TTATGGC670700.044.2314574
TTATCCC245950.044.199534
CTTATCT800850.044.1128043
TATGCGG196400.043.9204065
TTATGTC387650.043.895184
CTTATAG384650.043.7875253
TTATGTG256200.043.7217834
TATGCCG176100.043.6954465
TATGGCC219600.043.6744425
TTATGGG235950.043.5179524
Produced by FastQC (version 0.11.2)