FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_fast_93v3_t025.3410000000262e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_fast_93v3_t025.3410000000262e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6225823
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAAC80850.129862349122357No Hit
TTCGAGTGCTGAGTCAACAGCAGAGAGATGCTCCACAGGAGTTTGCTCTGC72150.11588829300158389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAGT5449950.044.972211
TCGAGTG2309300.044.8926242
TCGAGTC1795100.044.883382
CGAGTGC789900.044.7896423
CGAGTGT617500.044.785793
CGAGTCG447000.044.7747843
CGAGTGG688250.044.7540933
TCGAGTA916300.044.723472
CGAGTCT623600.044.6903533
CGAGTCC429050.044.676823
CGAGTCA305350.044.4336553
TCGAGTT449550.044.4007232
CTAGTCG53950.044.319053
CGAGTGA222650.044.248653
CGAGTAG322700.044.2263683
GAGTGCG182950.044.2115674
GAGTGCC330950.044.211334
GAGTCCC168050.044.1964264
GAGTCGC145350.044.1814234
CGAGTTG209850.044.0857623
Produced by FastQC (version 0.11.2)