FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_fast_93v3_t015.34100000002611.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_fast_93v3_t015.34100000002611.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7930056
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCCTGCTGAGTCAACAGCAGAGAGATGCTCCACAGGAGTTTGCTCTGC98150.1237696177681469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCT7716400.044.9138151
GTTCCTG3447250.044.7187082
GTTCCTA1211950.044.7177662
GTTCCTC2313400.044.7097742
TTCCTGC1277800.044.1806533
TCCTGGC479750.044.08644
CCTAGGC24200.044.056855
TCCTAGC180750.044.053274
TTCCTAG367300.044.0009233
TTCCTGG974550.043.982873
TCCTGCC506950.043.7664344
TTCCTGT923750.043.7547653
TCCTACG81550.043.74544
TTCCTAT327150.043.5911453
TCCTGCG330950.043.5865064
TCCTCGC185600.043.5080534
TTCCTAC303050.043.4801453
CCTGCCC137450.043.431685
TCCTCCG185950.043.389874
TTCCTCG590700.043.3685153
Produced by FastQC (version 0.11.2)