FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_fast_93v3_t010.34100000002604.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_fast_93v3_t010.34100000002604.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8522204
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATCTGCTGAGTCAACAGCAGAGAGATGCTCCACAGGAGTTTGCTCTGC94060.11037050978831298No Hit
CAGATCTGTGCCTTTTGGTAACGACTGATCGATAGATACGACCTTTAAAAC92000.10795329471108647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATCT8392250.044.758171
AGATCTC2607000.044.5703242
GATCTGC1370200.044.5667233
AGATCTA1285650.044.5479432
ATCTCGG273050.044.3342934
AGATCTG3752000.044.3138962
GATCTCG679150.044.1540373
GATCTCC727900.044.055213
GATCTGT961500.044.0261963
ATCTGCC571400.044.0167054
GATCTGG1050100.043.972173
GATCTAC321750.043.761943
GATCTAG396800.043.721243
ATCTCGC223650.043.707814
GATCTCT843900.043.56473
ATCTGGC551950.043.4976434
ATCTCCC274150.043.4590264
ATCTGCG357350.043.1786044
ATCTCCG216000.042.954484
GATCTCA408850.042.6252023
Produced by FastQC (version 0.11.2)