FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l08n01 carbon_fast_93v3_t005.341000000025f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l08n01 carbon_fast_93v3_t005.341000000025f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3070211
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAATTGTGCCTTTTGGTAACGACTGATCGATAGATACGACCTTTAAAAC89560.2917063354929026No Hit
GCCAATTCTCGCCTTTTTGTACTTTTGTATCTTTCAGCTGGCCGAAAGCTG55470.1806716215921316No Hit
GCCAATTGGCTCTTCTTCTTTTGTTTCTTCTTTCGCTGGTGCTGAACCTTG51710.16842490630122817No Hit
GCCAATTCCCGTATCTCTGAGTCAAATGCATTTTCGGTAGGCAGTGTCACC51500.16774091422381068No Hit
GCCAATTCCCGTTTATTGTAAGCTTCACAGGTTTTGCAGCCTCATACACAA49550.16138955921921977No Hit
GCCAATTGCCTTTTGGTAACGACTGATCGATAGATACGACCTTTAAAACGC46780.15236737800757016No Hit
GCCAATTGCCGGTCATTGGGAATTTCCTTCACTACTTCCTTCGGTTTCTTC42170.1373521233556912No Hit
GCCAATTGCCTTTTTGTACTTTTGTATCTTTCAGCTGGCCGAAAGCTGTAA41890.13644013391913454No Hit
GCCAATTATTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGGG38790.12634310801440032TruSeq Adapter, Index 20 (97% over 37bp)
GCCAATTGGCTCGCCTTTTTGTACTTTTGTATCTTTCAGCTGGCCGAAAGC37220.121229452959422No Hit
GCCAATTGCCGTCTGCTTCTCCTGCAAGGATCATACCTTGAGAAAGCTCTC36510.11891690831672481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAATT2975450.044.8335841
CCAATTA594750.044.5978742
CCAATTG1255800.044.3748442
CCAATTC883050.044.095882
CAATTAT255950.044.038293
CAATTGC458400.043.9078833
AATTGCC191150.043.727584
ATTCCCG40100.043.5843665
CAATTAG140500.043.5305373
CAATTCG226500.043.358743
CAATTCC247950.043.290993
AATTCGC72150.043.2720264
CAATTGT336900.043.1179583
ATTGCCC44150.043.0506865
AATTCCC95950.042.923734
AATTGCG113150.042.6608244
CAATTGG356500.042.633953
CCAATTT296550.042.4843332
TATTGGC132000.042.4285557
GCACACG127100.042.28588525
Produced by FastQC (version 0.11.2)