FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l07n01 g3_small3.34100000009202.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l07n01 g3_small3.34100000009202.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56887075
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG34194576.010955915733055Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG27060074.756804599287272Illumina Small RNA Adapter 2 (100% over 21bp)
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC21828963.8372442246327485RNA PCR Primer, Index 1 (100% over 31bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC21595663.79623315137929RNA PCR Primer, Index 1 (100% over 24bp)
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA10730291.8862439315081678RNA PCR Primer, Index 1 (100% over 23bp)
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA8898301.5642041711583872RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA7258071.2758732981085774RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT6332811.1132247527228285RNA PCR Primer, Index 1 (100% over 25bp)
GCACTGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG5945561.045151293154025Illumina Small RNA Adapter 2 (100% over 21bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG4709450.8278594039155642No Hit
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC4567440.8028959126479961RNA PCR Primer, Index 1 (100% over 24bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC3875950.6813410603375196No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA3471790.6102950450519735RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGCC3407150.5989321827497723RNA PCR Primer, Index 8 (100% over 51bp)
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG3374980.59327711962691No Hit
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG3237630.5691327951032814No Hit
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2858100.5024164100544807RNA PCR Primer, Index 1 (100% over 30bp)
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA2244250.39450964916020026RNA PCR Primer, Index 1 (100% over 22bp)
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA1944850.34187906479635316No Hit
CCTTGACACGGATCAGAGGCCAGTTCGATTCTGGCCTGGGACACCTGGAAT1938080.34068898778852663No Hit
CAGACTGTTAATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATG1785530.3138727030700735No Hit
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC1756640.3087942208313576No Hit
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA1682070.29568579505977416No Hit
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG1674050.29427598448329434No Hit
CCTTGACACGGATCAGAGGCCAGTTCGATTCTGGCCTGGGACACCATGGAA1592450.2799317771216045No Hit
ATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGG1559940.27421694646806855No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGTGGAATTCTCGGGTGCCAAG1412530.24830420618391788No Hit
GGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTC1358760.23885214699472598RNA PCR Primer, Index 1 (100% over 26bp)
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA1329750.23375257033341934RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTTGGAATTCTCGGGTGCCAAG1328650.23355920479300438No Hit
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC1310930.2304442617237747No Hit
GCACCGCTGGTCTAATGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACTC1161670.2042063157580171RNA PCR Primer, Index 1 (100% over 26bp)
TAAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTC1153890.20283869402671872No Hit
TAGCTGTGACGATATTAAATGCACCACCTAGCTGATTTACAGATGATTGCA1127490.19819792105676023No Hit
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG1087290.19113128948886896Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGG1082030.19020665063197573No Hit
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT984170.1730041490092433RNA PCR Primer, Index 8 (100% over 36bp)
GCCTGTGTAGCTCAACCGGATAGAGCATATGGAATTCTCGGGTGCCAAGGA957170.16825790392633125RNA PCR Primer, Index 1 (100% over 22bp)
ATCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGG907170.15946856118019778No Hit
GCTGGAGCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGC874370.15370275233873423No Hit
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG873810.15360431169997754No Hit
GCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGA872840.15343379845070257RNA PCR Primer, Index 1 (100% over 22bp)
TCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGG869460.15283963888106392No Hit
GCACTGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA854250.15016592081769012RNA PCR Primer, Index 1 (100% over 22bp)
GGGTGAGTAGTCTAGTGGTATGATTCCTGTGGAATTCTCGGGTGCCAAGGA849440.1493203860455121RNA PCR Primer, Index 1 (100% over 22bp)
GGGTGAGTAGTCTAGTGGTATGATTCTGGAATTCTCGGGTGCCAAGGAACT825870.1451770898749848RNA PCR Primer, Index 1 (100% over 25bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG813550.1430113958223375No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGTGGAATTCTCGGGTGCCAAGGAA810170.14241723625269886RNA PCR Primer, Index 1 (100% over 23bp)
TCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGG801660.14092129011730697No Hit
ACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCGG797720.14022868990891166No Hit
GGGACTTTAGCTCACCTGGGAGAGCGTGCGATGGAATTCTCGGGTGCCAAG789300.13874856459046278No Hit
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC777980.13675865739273815RNA PCR Primer, Index 5 (100% over 35bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCCTGGAATTCTCGGGTGCCAAG759940.1335874625299332No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA742400.1305041610945896RNA PCR Primer, Index 1 (100% over 23bp)
TCCGACATCGTTCATCGGTTAGGATAATGGAATTCTCGGGTGCCAAGGAAC686150.12061615050518945RNA PCR Primer, Index 1 (100% over 24bp)
AGCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGG675020.11865964280990014Illumina Small RNA Adapter 2 (100% over 21bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCTGGAATTCTCGGGTGCCAAGG658160.11569587643590394Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGGT643300.11308368377175308No Hit
CGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTG610050.10723877084557433RNA PCR Primer, Index 8 (100% over 38bp)
GCCCCAATAGTATATCGGTTAGAACACATTGGAATTCTCGGGTGCCAAGGA604350.10623678577251511RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTT602730.10595201106754039RNA PCR Primer, Index 8 (100% over 37bp)
TCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGAA590660.10383026372862378No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGTGGAATTCTCGGGTGCCAAGG569890.10017917075187993Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC10633100.045.0013541
CACCGCT10906650.044.8742372
TCCGACA3852100.044.842611
GCACTGC965200.044.8074651
TCCGATA1432150.044.780621
CCGACAT3904950.044.6839752
CCCCAAT728050.044.670472
ACCGCTG11241000.044.66213
CACTGCT990850.044.6436352
CCGATAT1465450.044.6157042
CGACATC4000050.044.588053
TACATCT2613950.044.5703161
TCGCGAT2760800.044.5421715
TTAATCG2848100.044.5410911
CGCGATG2738350.044.54018416
AATCGCG2834400.044.52596313
TAATCGC2840150.044.50187312
ATCGCGA2762100.044.44237514
GACATCG4004700.044.4329264
CGATATC1502150.044.426333
Produced by FastQC (version 0.11.2)