FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l07n01 g3_small2.341000000091e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l07n01 g3_small2.341000000091e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47747619
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG22588954.730906058373298Illumina Small RNA Adapter 2 (100% over 21bp)
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC19277954.037468339520762RNA PCR Primer, Index 1 (100% over 31bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG16152303.3828493102451875Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC15408093.2269860409165116RNA PCR Primer, Index 1 (100% over 24bp)
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA8289171.736038398061273RNA PCR Primer, Index 1 (100% over 23bp)
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA6470061.3550539556747323RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA5845551.2242599992263488RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT5468491.1452906164807926RNA PCR Primer, Index 1 (100% over 25bp)
GCACTGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG4894161.0250060845965954Illumina Small RNA Adapter 2 (100% over 21bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG4014350.8407434934085405No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC3650180.7644737217158409No Hit
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC3404230.7129632998034939RNA PCR Primer, Index 1 (100% over 24bp)
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA2624690.5497007086363825RNA PCR Primer, Index 1 (100% over 22bp)
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG2529790.5298253720253568No Hit
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG2461500.515523088177444No Hit
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA2267130.4748152991670643No Hit
CAGACTGTTAATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATG2123920.4448221805573175No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA1768140.37030956454603525RNA PCR Primer, Index 1 (100% over 22bp)
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1476220.30917143742811554RNA PCR Primer, Index 1 (100% over 30bp)
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA1353380.2834445001330852No Hit
TAGCTGTGACGATATTAAATGCACCACCTAGCTGATTTACAGATGATTGCA1322480.2769729732492001No Hit
ACATCAGTACTGGGCTGGGCGACTTCCCGGGAACGTGCGGTGCTTGCTTCT1291980.27058522017610975No Hit
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG1217660.2550200461304678No Hit
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC1216380.25475196993592497No Hit
CCTTGACACGGATCAGAGGCCAGTTCGATTCTGGCCTGGGACACCTGGAAT1166700.24434726263523215No Hit
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA1153760.24163717985602592RNA PCR Primer, Index 1 (100% over 23bp)
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC1069290.2239462453614703No Hit
GGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTC1042380.21831036224026165RNA PCR Primer, Index 1 (100% over 26bp)
ATGGGTGTGACAGTACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1042190.2182705696801342RNA PCR Primer, Index 1 (100% over 31bp)
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA992270.2078155980929646RNA PCR Primer, Index 2 (97% over 36bp)
TAAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTC914610.1915509127271875No Hit
ATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGG914490.19152578058394912No Hit
CCTTGACACGGATCAGAGGCCAGTTCGATTCTGGCCTGGGACACCATGGAA878370.18396100546919417No Hit
GGGTGAGTAGTCTAGTGGTATGATTCCTGTGGAATTCTCGGGTGCCAAGGA871090.18243632211273195RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTTGGAATTCTCGGGTGCCAAG867620.18170958430408854No Hit
GCACCGCTGGTCTAATGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACTC838830.17567996427214516RNA PCR Primer, Index 1 (100% over 26bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGTGGAATTCTCGGGTGCCAAG797990.16712665819001363No Hit
AGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGG788300.16509723762351375No Hit
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG781020.1635725542670515Illumina Small RNA Adapter 2 (100% over 21bp)
ATCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGG774710.16225102240176625No Hit
GCACTGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA774600.16222798460379773RNA PCR Primer, Index 1 (100% over 22bp)
GGGTGAGTAGTCTAGTGGTATGATTCTGGAATTCTCGGGTGCCAAGGAACT770120.1612897179228979RNA PCR Primer, Index 1 (100% over 25bp)
TCGGTCATAGTGTAATGGTTATCATTTAAGATGGAATTCTCGGGTGCCAAG744360.15589468450772384No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATATGGAATTCTCGGGTGCCAAGGA735860.154114491028338RNA PCR Primer, Index 1 (100% over 22bp)
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG708840.14845557010916083RNA PCR Primer, Index 2 (97% over 35bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCGATGGAATTCTCGGGTGCCAAG683790.14320923520814724No Hit
GCTGGAGCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGC667510.13979964110880586No Hit
CGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC664080.13908128068124193RNA PCR Primer, Index 4 (100% over 38bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCCTGGAATTCTCGGGTGCCAAG652440.13664346278711825No Hit
GCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGA651070.13635653748514664RNA PCR Primer, Index 1 (100% over 22bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA633200.13261394248789662RNA PCR Primer, Index 1 (100% over 23bp)
TCCGACATCGTTCATCGGTTAGGATAATGGAATTCTCGGGTGCCAAGGAAC631090.13217203563595495RNA PCR Primer, Index 1 (100% over 24bp)
TCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGAA624270.13074369216190654No Hit
CCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC623210.1305216915633008RNA PCR Primer, Index 4 (100% over 37bp)
TCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGG615260.12885668707375755No Hit
CATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC612500.1282786477792746RNA PCR Primer, Index 4 (100% over 37bp)
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG608880.12752049479158323No Hit
TCCTCCCTAACTCAGTTGGTTAGAGTGTCAGATGGAATTCTCGGGTGCCAA587280.1229967090086733No Hit
CTCATAATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAAT584290.12237049977298345No Hit
TCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGG555500.11634087974104006No Hit
TACATCTTTTTTAATCGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC548060.11478268686025998RNA PCR Primer, Index 1 (100% over 31bp)
TCGGTCATAGTGTAATGGTTATCATGTAAGATGGAATTCTCGGGTGCCAAG536510.11236371807356509No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG522560.10944210642210242No Hit
GTCCCAGTAGTATATTGGTTAGAATAGATCTGGAATTCTCGGGTGCCAAGG499830.10468165962369767Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGGCGGTGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA497860.10426907360553413RNA PCR Primer, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC7742800.044.9941671
CACCGCT7903800.044.8482252
TCCGACA2528450.044.779751
GCACTGC807950.044.771821
TCCGATA1059100.044.7560841
CACTGCT825450.044.6473272
CCCCAAT556800.044.632452
CCGATAT1077500.044.574612
CCGACAT2559400.044.5678562
ACCGCTG8151900.044.5676083
TACATCT2435200.044.5326421
CGACATC2622800.044.4675833
TTAATCG2659300.044.4604611
CGCGATG2471550.044.457716
TCGCGAT2494900.044.45627215
AATCGCG2619900.044.4444713
TAATCGC2653450.044.4296412
ATCGCGA2495600.044.41216714
CGATATC1104650.044.315333
TGCTGGT883350.044.2304655
Produced by FastQC (version 0.11.2)