FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l07n01 g3_small1.341000000091cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l07n01 g3_small1.341000000091cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56823267
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG26516454.6664775539921Illumina Small RNA Adapter 2 (100% over 21bp)
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC22315763.927222276748009RNA PCR Primer, Index 1 (100% over 31bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG18881323.3228149307219526Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC18203543.2035363260616463RNA PCR Primer, Index 1 (100% over 24bp)
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA9820661.7282814801901483RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA7082131.246343333268747RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC6796981.1961614245094354No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA6208421.092584134594021RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT5822081.0245943796226993RNA PCR Primer, Index 1 (100% over 25bp)
GCACTGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG5236300.9215063259210352Illumina Small RNA Adapter 2 (100% over 21bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG4767550.8390137089442605No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA3717550.6542302469162852RNA PCR Primer, Index 1 (100% over 22bp)
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC3457190.6084109877033294RNA PCR Primer, Index 1 (100% over 24bp)
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG3358700.5910782989651053No Hit
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG2975870.5237062487097055No Hit
TATCACTTGGGACTTTGAATCCCGAAATCCAGGTTCGACTCCTGGCGGGAC2650630.4664691313859163No Hit
ATCACTTGGGACTTTGAATCCCGAAATCCAGGTTCGACTCCTGGCGGGACC2451300.4313901909230245No Hit
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA2433150.42819607679368377No Hit
TAGCTGTGACGATATTAAATGCACCACCTAGCTGATTTACAGATGATTGCA1779730.31320444845242706No Hit
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1774490.31228229098478266RNA PCR Primer, Index 1 (100% over 30bp)
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA1756430.30910401543790156RNA PCR Primer, Index 1 (100% over 22bp)
CAGACTGTTAATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATG1606580.2827327756427662No Hit
CCTTGACACGGATCAGAGGCCAGTTCGATTCTGGCCTGGGACACCTGGAAT1334330.23482106370265546No Hit
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG1321010.23247695349864345No Hit
CCTTGACACGGATCAGAGGCCAGTTCGATTCTGGCCTGGGACACCATGGAA1315360.2314826424886834No Hit
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA1262710.22221707174985206No Hit
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC1226990.2159309143559099No Hit
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA1184290.20841638690010555RNA PCR Primer, Index 2 (100% over 36bp)
TCGGTCATAGTGTAATGGTTATCATTTAAGATGGAATTCTCGGGTGCCAAG1179540.20758046171474087No Hit
GGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTC1153450.20298903264396959RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC1102720.19406135166427513No Hit
TTGCTAATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGC1102030.1939399225320853No Hit
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA1077200.18957023361574757RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACTC1032120.18163686364601317RNA PCR Primer, Index 1 (100% over 26bp)
ATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGG1027960.18090476916788328No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT1011800.17806086369514798RNA PCR Primer, Index 2 (100% over 46bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTTGGAATTCTCGGGTGCCAAG1008300.1774449188217214No Hit
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG976320.17181694252109792Illumina Small RNA Adapter 2 (100% over 21bp)
TAAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTC963960.16964177719665433No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGTGGAATTCTCGGGTGCCAAG907460.15969866709705374No Hit
GCACTGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA883510.1554838443203204RNA PCR Primer, Index 1 (100% over 22bp)
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG881090.15505796243640832RNA PCR Primer, Index 2 (100% over 35bp)
ATCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGG864550.15214718294884383No Hit
TCCTACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG850090.14960245070034428Illumina Small RNA Adapter 2 (100% over 21bp)
TCACTTGGGACTTTGAATCCCGAAATCCAGGTTCGACTCCTGGCGGGACCA843950.1485219074081045No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATATGGAATTCTCGGGTGCCAAGGA827470.14562168697551303RNA PCR Primer, Index 1 (100% over 22bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCGATGGAATTCTCGGGTGCCAAG793630.13966637997072573No Hit
TCCTCCCTAACTCAGTTGGTTAGAGTGTCAGATGGAATTCTCGGGTGCCAA786790.13846264770380062No Hit
GGGTGAGTAGTCTAGTGGTATGATTCCTGTGGAATTCTCGGGTGCCAAGGA784730.1381001201497267RNA PCR Primer, Index 1 (100% over 22bp)
ACATCAGTACTGGGCTGGGCGACTTCCCGGGAACGTGCGGTGCTTGCTTCT780640.13738034456906534No Hit
CGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG778590.13701957685748692RNA PCR Primer, Index 2 (100% over 38bp)
AGCGCAAGGTCGCCGGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGG764930.13461563200862775No Hit
CATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT756780.13318136037479153RNA PCR Primer, Index 2 (100% over 37bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCCTGGAATTCTCGGGTGCCAAG729740.1284227462669473No Hit
GGGTGAGTAGTCTAGTGGTATGATTCTGGAATTCTCGGGTGCCAAGGAACT723380.12730348643980643RNA PCR Primer, Index 1 (100% over 25bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG696780.12262230540176439No Hit
TCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGG693900.12211547076305908No Hit
TCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGAA693190.12199052194587826No Hit
TCGGTCATAGTGTAATGGTTATCATATAAGATGGAATTCTCGGGTGCCAAG672140.11828605349284123No Hit
CTCATAATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAAT671300.11813822672321886No Hit
GCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGA655540.11536471495030372RNA PCR Primer, Index 1 (100% over 22bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT653140.11494235275138263RNA PCR Primer, Index 2 (100% over 46bp)
ATGGGTGTGACAGTACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTC648650.1141521834709011RNA PCR Primer, Index 1 (100% over 31bp)
CCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT636250.11196997877647549RNA PCR Primer, Index 2 (100% over 37bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA616410.1084784512653945RNA PCR Primer, Index 1 (100% over 23bp)
TCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGG613710.10800329379160828No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGTGGAATTCTCGGGTGCCAAGGAA612550.10779915206212975RNA PCR Primer, Index 1 (100% over 23bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCTGGAATTCTCGGGTGCCAAGG612480.10778683316466124Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGTCATAGTGTAATGGTTATCATGTAAGATGGAATTCTCGGGTGCCAAG608690.10711985285886501No Hit
GCTGGAGCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGC603030.10612378200640946No Hit
AAGTCTCTAAGCAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT589630.10376559306243338RNA PCR Primer, Index 2 (100% over 37bp)
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG589210.10369167967762219No Hit
GGTCCCGTAGTGTAGCGGTTATCACTTGGTGGAATTCTCGGGTGCCAAGGA581130.10226972694125454RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC8915200.044.985121
GCACTGC875250.044.778481
TCCGACA2769900.044.7590831
TCCGATA1012200.044.674681
CACCGCT9183950.044.5956462
CCCCAAT728850.044.5929832
TACATCT2698300.044.5724751
CACTGCT901400.044.56132
TCGCGAT2863100.044.520815
CGCGATG2840450.044.5171116
TTAATCG2951450.044.5093311
AATCGCG2938450.044.4587913
TAATCGC2944650.044.45878212
ATCGCGA2860750.044.42721614
CCGACAT2830750.044.3833052
ACCGCTG9474900.044.3754353
CGACATC2896550.044.318063
GCATCGC151150.044.2708851
CTTTTTT3051250.044.2318236
CCGATAT1041300.044.2038732
Produced by FastQC (version 0.11.2)