FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l06n01 ah8151b-042415.34100000009154.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l06n01 ah8151b-042415.34100000009154.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10220750
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1906741.8655578113152167TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG224200.042.60942511
CACACGT225250.042.36961412
ACGTCTG226800.042.00959815
ACACGTC227100.041.9947513
GATCGGA230200.041.983651
CACGTCT227750.041.9623414
AGCACAC229900.041.6314710
GAGCACA230450.041.5906759
TATCTCG219050.041.25696639
GTATGCC217450.041.19119645
AGAGCAC233800.041.0909548
CGTCTGA231850.041.08487716
GAACTCC230650.040.93346421
CAGTCAC229100.040.802827
TCGGAAG237250.040.7395323
CGATGTA220600.040.7386334
CCGATGT221250.040.73816733
CCAGTCA230250.040.69692226
TCCAGTC231400.040.58216525
AGTCACC228300.040.36317428
Produced by FastQC (version 0.11.2)