FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l06n01 ah8151a-042415.34100000009147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l06n01 ah8151a-042415.34100000009147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28762631
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG716052724.895243414971322TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTCAACAATCTCGTATG291350.10129462774111311TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACAACAATCTCGTATG290670.10105820986960477TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8152900.044.8888971
TCGGAAG8167500.044.6784063
ATCGGAA8190200.044.642832
GCACACG8104650.044.5410811
GAGCACA8144700.044.534769
CGGAAGA8192550.044.518464
AGAGCAC8154100.044.5079738
AGCACAC8128800.044.49540710
CACACGT8123700.044.43288812
ACACGTC8116550.044.41169413
GAAGAGC8214150.044.3388066
CACGTCT8111600.044.33694514
ACGTCTG8122800.044.30519515
GGAAGAG8246150.044.296815
CGTCTGA8133100.044.21838816
AAGAGCA8235850.044.1954047
GTCTGAA8168450.044.10668617
TCGTATG8038500.044.04433445
TCTGAAC8143750.044.0358418
GAACTCC8095200.043.96811721
Produced by FastQC (version 0.11.2)