FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l06n01 ah8148b-042415.3410000000913a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l06n01 ah8148b-042415.3410000000913a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12190957
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC301230.24709298868005194TruSeq Adapter, Index 7 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA157870.1294976268064927No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC131350.10774379730811945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG55250.034.6053911
ACACGTC56450.034.02886213
CACGTCT57950.033.45774514
CACACGT57800.033.19502312
ACGTCTG58750.033.04057315
CGTCTGA59850.032.4333116
AGCACAC62500.030.9510610
GAGCACA63100.030.7993439
AGAGCAC64600.030.3279278
GATCGGA64750.030.2168221
GAACTCC63900.030.20403121
CAGTCAC61450.029.58182327
TCCAGTC64750.029.29062525
TCGGAAG69150.028.7881363
CCAGTCA64950.028.6462126
AGTCACC61250.028.61299328
CTCGGGG21650.028.57060416
GTCTGAA68350.028.39990617
GTATGCC55400.027.3720345
GTCTCCC28900.027.03804642
Produced by FastQC (version 0.11.2)