FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l06n01 ah8148a-042415.3410000000912d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l06n01 ah8148a-042415.3410000000912d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13225720
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2566531.940559757805246TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG305050.043.40303811
GATCGGA308950.043.1607631
ACACGTC307250.043.00343313
CACACGT308200.042.8999112
CACGTCT309200.042.7529214
ACGTCTG309650.042.7200115
CGTCTGA310550.042.55998616
AGCACAC314150.042.20990410
GAGCACA316100.042.0064439
GAACTCC314950.041.79589521
GTATGCC297550.041.77522745
AGAGCAC318450.041.7317778
CAGTCAC313000.041.60105527
TCCAGTC316050.041.52718425
TCGGAAG323500.041.311033
CCAGTCA318000.041.18763726
AGTCACC309000.041.1271328
GTCACCT309100.040.94363829
ACTCCAG322100.040.7676723
CGTATGC304950.040.74802844
Produced by FastQC (version 0.11.2)