FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l06n01 ah8115b-042415.34100000009197.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l06n01 ah8115b-042415.34100000009197.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11799148
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC8579797.2715335039445215TruSeq Adapter, Index 6 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA1032760.8752835374215155No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC901360.7639195643617658No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC176580.14965487338577327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1010050.044.3481
GCACACG1000750.044.2248911
TCGGAAG1012050.044.1863753
CACACGT1003550.044.0516312
ACACGTC1003100.044.04242313
GAGCACA1010600.044.018479
AGCACAC1008050.043.97361810
CACGTCT1002700.043.9728814
ATCGGAA1018550.043.9596332
ACGTCTG1005000.043.91943415
CGGAAGA1021700.043.7602234
CGTCTGA1009200.043.71659516
AGAGCAC1018300.043.709928
GTCACGC984050.043.6670729
GTATGCC969750.043.59296845
GAACTCC1003100.043.5563621
AGTCACG991100.043.5555828
GTCTGAA1015650.043.52312517
TCACGCC978200.043.5063630
TCCAGTC999900.043.4433425
Produced by FastQC (version 0.11.2)