FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l06n01 ah8115a-042415.3410000000918a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l06n01 ah8115a-042415.3410000000918a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20429511
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG7542763.6920903295237952TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA927200.043.7803841
GCACACG913150.043.6947711
CGTCCCG850850.043.44270735
GTCCCGA838900.043.27462836
CCCGTCC867250.043.1742833
ACACGTC924750.043.17014313
ACGTCTG921700.043.1607415
CACGTCT923950.043.123714
CACACGT926200.043.10478212
GAGCACA930750.043.1001179
AGCACAC928750.043.0984710
ACCCGTC880150.042.98172432
TCACCCG890900.042.9487830
AGAGCAC936200.042.9309048
CGTCTGA925800.042.93072516
CCCGATC845500.042.92312638
CACCCGT883950.042.90371731
CCGTCCC864850.042.88348834
TCGGAAG948000.042.7501753
TCCCGAT851650.042.61678337