FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l06n01 ah8104b-042415.3410000000917d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l06n01 ah8104b-042415.3410000000917d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21568401
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1966560.911778300115989TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG245300.041.44869611
ACACGTC250400.040.4693713
CACACGT252400.040.25545512
ACGTCTG252750.040.13582615
CACGTCT254000.039.8763214
AGCACAC256850.039.81254210
GATCGGA258900.039.8026241
GTCACAC243350.039.7492829
GAGCACA259450.039.4569179
GATCTCG243350.039.06882539
CGTCTGA260350.038.97284316
CACACAG249050.038.8801931
GAACTCC259700.038.83192421
CAGTCAC255900.038.4809527
GTATGCC251600.038.2283145
TCGGAAG271300.038.097873
TCCAGTC263400.037.7357925
AGAGCAC272450.037.6567888
AGTCACA261950.037.5737928
CACAGTG252550.037.54224433
Produced by FastQC (version 0.11.2)