FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l06n01 ah8104a-042415.34100000009160.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l06n01 ah8104a-042415.34100000009160.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25707763
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG9176793.569657149865587TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1108000.043.719611
GCACACG1093900.043.6327211
ACACGTC1103300.043.27703513
CACACGT1104300.043.27417412
ACGTCTG1104850.043.16391415
CACGTCT1106300.043.09302514
GAGCACA1115350.043.0033389
CGTCTGA1108550.042.97520416
AGCACAC1112450.042.96985610
TCGGAAG1127550.042.8726123
AGAGCAC1120300.042.8554958
GAACTCC1109400.042.49225221
ATCGGAA1141550.042.4336932
TCCAGTC1109050.042.2679925
CAGTCAC1110300.042.22615427
GTCTGAA1133500.042.11871717
TCGTATG1103400.042.05675545
AGTCACA1115400.042.0562228
CCAGTCA1113750.042.02902226
ACTCCAG1117400.041.9899823