FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l06n01 ah7609b-042415.341000000090f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l06n01 ah7609b-042415.341000000090f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10139033
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA776530.7658817167278181No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC625480.6169030123484163No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG447320.44118605788145676TruSeq Adapter, Index 19 (97% over 40bp)
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC151160.14908719598801976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGTC91000.041.79665838
CTCGGGG73200.041.6356216
CTGACCC86550.041.1380640
CCTCGGG74050.041.12731615
TCGGGGA92750.041.10480517
CAGGCGG93150.040.8750536
TAAGCCG87550.040.79727616
GCGGTCT94150.040.54176339
CCTGACC88000.040.4834139
CCAGGGA88100.040.470785
CCCAGGC94100.040.3997734
CCCTGAC88450.040.22652438
CGGTCTC94850.040.1974640
CCAGGCG94650.040.100635
GCACACG63700.040.0789911
TCCAGGG89250.039.9235154
GTCTCCC95700.039.88493342
AGGCGGT95900.039.7068537
AGTCACG59000.039.50145728
GGTCTCC97250.039.27374641