FastQCFastQC Report
Wed 29 Apr 2015
C5726ACXX l06n01 ah3337b-042415.34100000009110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5726ACXX l06n01 ah3337b-042415.34100000009110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21478674
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC2650921.2342102682875116TruSeq Adapter, Index 4 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA241780.11256747041274522No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC214980.10008997762152358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG319800.042.84214411
ACACGTC322950.042.39551513
ACGTCTG323200.042.37605315
CACGTCT325950.042.0045314
GATCGGA330100.041.985421
CACACGT326900.041.91755312
CGTCTGA329900.041.49497216
AGCACAC331800.041.33337410
GAGCACA338100.040.636379
TCGGAAG344150.040.2563673
AGAGCAC342850.040.132428
GAACTCC338950.040.0727221
ACTGACC321300.040.07133532
CAGTCAC335300.040.06941627
GTATGCC314800.039.9862245
CCAGTCA340800.039.56202726
TCCAGTC342800.039.46246725
CGTATGC320100.039.3125444
CACTGAC333400.039.2441831
CGGAAGA354700.039.0399784
Produced by FastQC (version 0.11.2)